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Tag Archives: online databases

miRandb – a resource of online services for miRNA research

January 25, 2017 Leave a comment 5,220 Views

rna-seq

Recent discovery of thousands of small and large noncoding RNAs, in parallel to technical improvements enabling scientists to study the transcriptome in much higher depth, has resulted in massive data generation. This burst of information prompts the development of easily ...

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Recent RNA-SEQ Pubs

Mapping of a major QTL controlling plant height using a high-density genetic map and QTL-seq methods based on whole-genome resequencing in Brassica napus
9 April 2021
De novo assembly of the freshwater prawn Macrobrachium carcinus brain transcriptome for identification of potential targets for antibody development
9 April 2021
Cold stress triggers premature fruit abscission through ABA-dependent signal transduction in early developing apple
9 April 2021
(1)H NMR Metabolomics and Full-Length RNA-Seq Reveal Effects of Acylated and Nonacylated Anthocyanins on Hepatic Metabolites and Gene Expression in Zucker Diabetic Fatty Rats
9 April 2021
VvHDZ28 positively regulate SA biosynthesis during seed abortion in Thompson Seedless
9 April 2021
Nanopore RNA Sequencing Analysis
9 April 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    NanoSINC-seq dissects the isoform diversity in subcellular compartments of single cells

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