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Tag Archives: osmotic stress

Meet the latest RNAs – DoGs – downstream of gene containing transcripts

July 21, 2015 Leave a comment 3,609 Views

rna-seq

Pervasive transcription of the human genome generates RNAs whose mode of formation and functions are largely uncharacterized. Now researchers from Yale University School of Medicine have discovered yet another type of RNA: DoGs, which stands for – downstream of gene containing ...

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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