RNA sequencing using next-generation sequencing technologies (NGS) is currently the standard approach for gene expression profiling, particularly for large-scale high-throughput studies. NGS technologies comprise high throughput, cost efficient...
Read More »A comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome
The number of studies using third-generation sequencing utilizing Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) is rapidly increasing in many different research areas. Among them, plant full-length single-molecule transcriptome studies have mostly used PacBio sequencing, whereas ONT is rarely ...
Read More »A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes
A platform for highly parallel direct sequencing of native RNA strands was recently described by Oxford Nanopore Technologies, but despite initial efforts it remains crucial to further investigate the technology for quantification of complex transcriptomes. Now a team led by ...
Read More »Highly parallel direct RNA sequencing on an array of nanopores
Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due...
Read More »London Calling 2018
A conference hosted by Oxford Nanopore Technologies. 24th – 25th May Old Billingsgate, London Delegate rates: NCM offer: £599* Limited rate: £699** Poster rate: £749** Standard rate: £999** Pre-meeting workshop: £400 The pre-meeting workshop is available to members of the ...
Read More »Benchmarking of the Oxford Nanopore MinION sequencing for quantitative and qualitative assessment of cDNA populations
To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, researchers from McGill University sequenced cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts ...
Read More »Highly parallel direct RNA sequencing on an array of nanopores
Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing — information ...
Read More »Researchers and public health officials use RNA-Seq to track transmission of epidemic viruses
(IEEE Spectrum) -Using a genome sequencer smaller than a stapler, geneticists have demonstrated the role they can play in combating outbreaks of infectious disease. An eight-month experiment in Guinea during the tail end of the Ebola outbreak, described today in ...
Read More »RNA-Seq Blog – Poll Results
We asked: Will the error rate issues with nanopore sequencing eventually be solved? How interesting that almost a third of you believe their are problems that cannot be overcome, even though there are several companies devoting significant resources toward development ...
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