Tag Archives: pacbio

RNA sequencing using next-generation sequencing technologies (NGS) is currently the standard approach for gene expression profiling, particularly for large-scale high-throughput studies. NGS technologies comprise high throughput, cost efficient...

The number of studies using third-generation sequencing utilizing Pacific Biosciences (PacBio) and Oxford Nanopore Technologies (ONT) is rapidly increasing in many different research areas. Among them, plant full-length single-molecule transcriptome studies have mostly used PacBio sequencing, whereas ONT is rarely ...

Significant Enhancements to Sequel System Enable >99% Accuracy for Single-Molecule Reads up to 15 kb; Throughput Increases by 2- to 4-Fold Pacific Biosciences of California, Inc. a provider of high-quality sequencing of genomes, transcriptomes and epigenomes, today announced major enhancements ...

Pacific Biosciences of California, Inc. today announced a new version of Sequel® Software (V5.1) and a new polymerase. Combined, these enhancements increase throughput and the overall...

The Isoform Sequencing (Iso - Seq™) method employs long reads to sequence transcript isoforms from the 5’ end to their poly - A tails, eliminating the need for transcript reconstruction and inference. These long reads result in complete, unambiguous...

In this study, researchers at Nankai University introduced a general framework to use PacBio full-length transcriptome sequencing for the investigation of mitochondrial RNAs. As a result, they produced the first full-length human mitochondrial transcriptome based on the public PacBio data ...

A series of recent peer-reviewed scientific publications highlights the growing use of Single Molecule, Real-Time (SMRT®) Sequencing to annotate plant and animal genomes. These reports from the genomics community show that PacBio sequencing consistently detects more genes, alternative splicing events, ...

Alternative splicing (AS) is a major source of transcript and proteome diversity, but examining AS in species without well-annotated reference genomes remains difficult. Research on both human and mouse has demonstrated the advantages of using Iso-Seq™ data for isoform-level transcriptome ...

To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, researchers from McGill University sequenced cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts ...

The majority of human genes are alternatively spliced, making it possible for most genes to generate multiple proteins. Alternative splicing has been shown to play a role in human cancer, muscular dystrophy, Alzheimer’s, and many other diseases. Understanding these diseases ...