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Tag Archives: PANDORA-seq

PANDORA sequencing method can detect once-undetectable small RNAs

12 days ago Leave a comment 621 Views

rna-seq

A team led by a biomedical scientist at the University of California, Riverside, has developed a new RNA-sequencing method— “Panoramic RNA Display by Overcoming RNA Modification Aborted Sequencing,” or PANDORA-seq — that can help discover numerous...

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Recent RNA-SEQ Pubs

Detection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species
18 April 2021
RNA-seq reveals plant virus composition and diversity in alfalfa, thrips, and aphids in Beijing, China
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Applications of single-cell and bulk RNA sequencing in onco-immunology
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Babaodan controls excessive immune responses and may represent a cytokine-targeted agent suitable for COVID-19 treatment
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Loss of claudin-3 impairs hepatic metabolism, biliary barrier function and cell proliferation in the murine liver
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A single cell atlas of human cornea that defines its development, limbal progenitor cells and their interactions with the immune cells
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    scGNN – a novel graph neural network framework for single-cell RNA-Seq analyses

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