Tag Archives: pathway analysis

Moving RNA-Seq Forward – Challenges Ahead

rna-seq

Next-generation sequencing technologies have revolutionarily advanced sequence-based research with the advantages of high-throughput, high-sensitivity, and high-speed. RNA-seq is now being used widely for uncovering multiple facets of transcriptome to facilitate the biological applications. However, the large-scale data analyses associated with ...

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PathwaySeq – Pathway analysis for RNA-Seq data

rna-seq

A variety of pathway/gene-set approaches have been proposed to provide evidence of higher-level biological phenomena in the association of expression with experimental condition or clinical outcome. Among these approaches, it has been repeatedly shown that resampling methods are far preferable ...

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The statistical universe is a single patient – pathway analysis from “N-of-1” RNA-Seq data

rna-seq

The emergence of precision medicine allowed the incorporation of individual molecular data into patient care. Indeed, DNA sequencing predicts somatic mutations in individual patients. However, these genetic features overlook dynamic epigenetic and phenotypic response to therapy. Meanwhile, accurate personal transcriptome ...

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Next Gen Sequencing or Microarray: How to Make the Choice

rna-seq

from BitsizeBio by Kristin Harper You’re about to start that big project you’ve been dreaming of for years. You’ve identified a potential miracle compound and want to figure out how it affects gene expression. But how are you going to ...

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RNA-Seq with iReport and InSilico DB

rna-seq

RNA-Seq, or transcriptome sequencing, continues to be an exciting way to explore gene expression using next-generation sequencing (NGS). But for many researchers, the interpretation of RNA-Seq data remains a daunting task because they are using spreadsheets to read transcript names ...

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