Tag Archives: PCR amplification

In ecological studies microbial diversity is nowadays mostly assessed via the detection of phylogenetic marker genes such as 16S ribosomal RNA. However, PCR amplification of these marker genes produces a significant amount of artificial sequences often referred to as chimeras. ...

Researchers at Stanford University present a simple molecular indexing method for quantitative targeted RNA sequencing, in which mRNAs of interest are selectively captured from complex cDNA libraries and sequenced to determine their absolute concentrations. cDNA fragments are individually labeled so ...