CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

December 23, 2014 Leave a comment 5,070 Views

In ecological studies microbial diversity is nowadays mostly assessed via the detection of phylogenetic marker genes such as 16S ribosomal RNA. However, PCR amplification of these marker genes produces a significant amount of artificial sequences often referred to as chimeras. ...

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

January 27, 2014 Leave a comment 8,240 Views

Researchers at Stanford University present a simple molecular indexing method for quantitative targeted RNA sequencing, in which mRNAs of interest are selectively captured from complex cDNA libraries and sequenced to determine their absolute concentrations. cDNA fragments are individually labeled so ...

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Tag Archives: PCR amplification

CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

scRNA-Seq analysis reveals that the cells regulating blood stem cell maintenance are more diverse than expected

Kodoja – a workflow for virus detection in plants using k-mer analysis of RNA-sequencing data

Live-seq – sequencing a cell without killing it

CellRegMap – a statistical framework for mapping context-specific regulatory variants using scRNA-seq

TGIRT sequencing reveals abundant non-microRNA-small RNAs in BLCA tumor samples

totRNAseq – high-throughput muscle fiber typing from RNA sequencing data

THRONE – a new approach for accurate prediction of human RNA N7-methylguanosine sites