CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

December 23, 2014 Leave a comment 5,287 Views

In ecological studies microbial diversity is nowadays mostly assessed via the detection of phylogenetic marker genes such as 16S ribosomal RNA. However, PCR amplification of these marker genes produces a significant amount of artificial sequences often referred to as chimeras. ...

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

January 27, 2014 Leave a comment 8,361 Views

Researchers at Stanford University present a simple molecular indexing method for quantitative targeted RNA sequencing, in which mRNAs of interest are selectively captured from complex cDNA libraries and sequenced to determine their absolute concentrations. cDNA fragments are individually labeled so ...

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Tag Archives: PCR amplification

CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

DirectRMDB – a database of post-transcriptional RNA modifications unveiled from direct RNA sequencing technology

Predicting response to immune checkpoint blockade in NSCLC with tumour-only RNA-seq

MATQ_Drop – Multiple-Annealing-and-Tailing-based Quantitative scRNA-seq in Droplets

Featured RNA-Seq Job – Research Technician II – Single Cell RNA Sequencing

IMMUcan scDB – a fully integrated scRNA-seq database exclusively dedicated to human cancer

Correspondence analysis for dimension reduction, batch integration, and visualization of single-cell RNA-seq data

Mutant KRAS signaling drives alternative splicing in lung cancer cells