CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

December 23, 2014 Leave a comment 4,582 Views

In ecological studies microbial diversity is nowadays mostly assessed via the detection of phylogenetic marker genes such as 16S ribosomal RNA. However, PCR amplification of these marker genes produces a significant amount of artificial sequences often referred to as chimeras. ...

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

January 27, 2014 Leave a comment 7,767 Views

Researchers at Stanford University present a simple molecular indexing method for quantitative targeted RNA sequencing, in which mRNAs of interest are selectively captured from complex cDNA libraries and sequenced to determine their absolute concentrations. cDNA fragments are individually labeled so ...

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Tag Archives: PCR amplification

CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

Penn scientists have developed a new method for tracing the lineage and gene expression patterns of metastatic cancer at the single-cell level

New technique visualizes all gene expression from a tissue

LACE-seq – global profiling of RNA-binding protein target sites

Researchers publish first transcriptomic study of decompression sickness

Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models

Bias in RNA-seq Library Preparation

Identifying differentially expressed circular RNAs associated with DNA methylation change