CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

December 23, 2014 Leave a comment 4,720 Views

In ecological studies microbial diversity is nowadays mostly assessed via the detection of phylogenetic marker genes such as 16S ribosomal RNA. However, PCR amplification of these marker genes produces a significant amount of artificial sequences often referred to as chimeras. ...

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

January 27, 2014 Leave a comment 7,868 Views

Researchers at Stanford University present a simple molecular indexing method for quantitative targeted RNA sequencing, in which mRNAs of interest are selectively captured from complex cDNA libraries and sequenced to determine their absolute concentrations. cDNA fragments are individually labeled so ...

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Tag Archives: PCR amplification

CATCh – an ensemble classifier for chimera detection in 16S rRNA sequencing studies

Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

NIH scientists build a cellular blueprint of multiple sclerosis lesions

RNA sequencing provides insights into the cellular and molecular changes in male infertility

The MAQC Society announces publication of a collection of next-gen sequencing best practice articles

New research provides useful genetic resource on farm-raised kuruma shrimp

Rutgers neurologist explores link between COVID and ‘Brain Fog,’ dementia with MRI and RNA sequencing

Upcoming Webinar – Impact of SARS-CoV-2 Infection on Host Gene Expression Across Multiple Tissues at Single Cell Resolution

Tissue heterogeneity as a widespread phenomenon in publicly available gene expression datasets