Tag Archives: pcr

Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference-based alignment. ...

Rare cell subtypes can profoundly impact the course of human health and disease, yet their presence within a sample is often missed with bulk molecular analysis. Single-cell analysis tools such as FACS, FISH-FC and single-cell barcode-based sequencing can investigate cellular ...

PCR clonal artefacts originating from NGS library preparation can affect both genomic as well as RNA-Seq applications when protocols are pushed to their limits. In RNA-Seq however the artifactual reads are not easy to tell apart from normal read duplication ...

High-throughput primer design is routinely performed in a wide number of molecular applications including genotyping specimens using traditional PCR techniques as well as assembly PCR, nested PCR, and primer walking experiments. Batch primer design is also required in validation experiments ...

from the Virology Blog By Vincent Racaniello The first immortal human cell line ever produced, HeLa, originated from a cervical adenocarcinoma taken from Henrietta Lacks. The cell line grew so well that it was used in many laboratories and soon ...

Position – Research Technician C Institution – Yeshiva University (Eisenstein, WI) Description Epigenomics Facility is searching for a Research Technician C to work with DNA and RNA for Resequencing, ChIP-seq, RNA-seq, small RNA-seq and HELP-tagging and bisulfite sequencing assays on ...

Wednesday, 30 April 2014 RNA-Seq 10:45 Peripheral Whole Blood Transcriptomes of Asthmatic Individuals Undergoing Allergen Inhalation Challenge Scott Tebbutt, Associate Professor, University of British ColumbiaAllergen inhalation challenge can be a suitable model to explore the dynamics of immune cells and ...