Tag Archives: Peking University

Single-cell transcriptome analysis through next-generation sequencing (single-cell RNA-seq) has been used broadly to address important biological questions. It has proved to be very powerful, and many exciting new biological discoveries have been achieved in the last decade. Its application has ...

Prevalent single cell RNA amplification and sequencing chemistries mainly focus on polyadenylated RNAs in eukaryotic cells by using oligo(dT) primers for reverse transcription. Researchers from Peking University have developed a new RNA amplification method, “easier-seq”, to reverse transcribe and amplify ...

Estimation of gene or isoform expression is a fundamental step in many transcriptome analysis tasks, such as differential expression analysis, eQTL (or sQTL) studies, and biological network construction. RNA-seq technology enables us to monitor the expression on genome-wide scale at ...

RNA-Seq is a powerful new technology to comprehensively analyze the transcriptome of any given cells. An important task in RNA-Seq data analysis is quantifying the expression levels of all transcripts. Although many methods have been introduced and much progress has ...

Single-cell whole-transcriptome analysis is a powerful tool for quantifying gene expression heterogeneity in populations of cells. Many techniques have, thus, been recently developed to perform transcriptome sequencing (RNA-Seq) on individual cells. To probe subtle biological variation between samples with limiting ...

from Peking University Single-cell genome and transcriptome sequencing methods are generating a fresh wave of biological insights into development, cancer and neuroscience. Kelly Rae Chi reports. Conceiving a child is an emotionally painful and exhausting process for those who struggle ...