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Tag Archives: PIWI-piRNA

Deep sequencing and high-throughput analysis of PIWI-associated small RNAs

May 31, 2017 Leave a comment 3,449 Views

Small RNAs are now known to be major regulatory factors of gene expression. Emerging methods based on deep-sequencing have enabled the analysis of small RNA expression in a high-throughput manner, leading to the identification of large numbers of small RNAs ...

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    An RNA-Seq framework for profiling target selectivity and physiological effects of antisense peptide nucleic acids (PNAs)

    27 mins ago
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    Single cell sequencing unravels molecular identity of rare renal cell types

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