Dolomite Bio, a leading provider of high throughput single cell technology, launches a new application note demonstrating single cell RNA Sequencing of plant leaf protoplasts on the Nadia Innovate The Nadia Instrument exhibits unprecedented sample type and size compatibility, allowing ...
Read More »PcircRNA_finder – circRNA prediction in plants
Recent studies reveal an important role of non-coding circular RNA (circRNA) in the control of cellular processes. Because of differences in the organization of plant and mammal genomes, the sensitivity and accuracy of circRNA prediction programs using algorithms developed for ...
Read More »The potential of single-cell transcriptomics in plants
Single-cell RNA-seq works as efficiently in plant cells as in animal cells. Noise profiles are well understood and an early set of analytical approaches is now capable of extracting information not previously possible in pooled samples. The biggest technical challenges ...
Read More »An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues
Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA ...
Read More »How to get high-quality RNA from chemically complex plants
Ask any molecular plant biologist about RNA extractions and you might just open up the floodgates to the woes of troubleshooting. RNA extraction is a notoriously tricky and sensitive lab procedure. New protocols out of the University of Florida are ...
Read More »Penn Scientists Identify Patterns of RNA Regulation in the Nuclei of Plants
When the human genome was first sequenced, experts predicted they would find about 100,000 genes. The actual number has turned out to be closer to 20,000, just a few thousand more than fruit flies have. The question logically arose: how ...
Read More »Comparisons of computational methods for differential alternative splicing detection using RNA-seq
Alternative Splicing (AS) as a post-transcription regulation mechanism is an important application of RNA-seq studies in eukaryotes. A number of software and computational methods have been developed for detecting AS. Most of the methods, however, are designed and tested on ...
Read More »MTide – an integrated tool for the identification of miRNA-target interaction in plants
Small RNA sequencing and degradome sequencing (also known as parallel analysis of RNA ends) have provided rich information on the microRNA (miRNA) and its cleaved mRNA targets on a genome-wide scale in plants, but no computational tools have been developed ...
Read More »Comprehensive Analysis of Alternative Splicing by Strand-Specific RNA-Seq
Digitalis purpurea (D. purpurea) is one of the most important medicinal plants and is well known in the treatment of heart failure because of the cardiac glycosides that are its main active compounds. However, in the absence of strand specific ...
Read More »Genomic-scale exchange of mRNA between a parasitic plant and its hosts
Movement of RNAs between cells of a single plant is well documented, but cross-species RNA transfer is largely unexplored. Cuscuta pentagona (dodder) is a parasitic plant that forms symplastic connections with its hosts and takes up host messenger RNAs (mRNAs). ...
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