High-throughput single-cell RNA-seq methods assign limited unique molecular identifier (UMI) counts as gene expression values to single cells from shallow sequence reads and detect limited gene counts. RIKEN institute researchers have developed a high-throughput single-cell RNA-seq method, Quartz-Seq2, to overcome ...
Read More »PAT-Seq – polyA-tagging and sequencing for tissue-specific expression analysis changes in an in vivo setting
Tissue-specific RNA plasticity broadly impacts the development, tissue identity and adaptability of all organisms, but changes in composition, expression levels and its impact on gene regulation in different somatic tissues are largely unknown. Now, researchers at Arizona State University have developed ...
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