Quartz-Seq2 – a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads

March 13, 2018 Leave a comment 4,904 Views

High-throughput single-cell RNA-seq methods assign limited unique molecular identifier (UMI) counts as gene expression values to single cells from shallow sequence reads and detect limited gene counts. RIKEN institute researchers have developed a high-throughput single-cell RNA-seq method, Quartz-Seq2, to overcome ...

PAT-Seq – polyA-tagging and sequencing for tissue-specific expression analysis changes in an in vivo setting

January 30, 2015 Leave a comment 5,134 Views

Tissue-specific RNA plasticity broadly impacts the development, tissue identity and adaptability of all organisms, but changes in composition, expression levels and its impact on gene regulation in different somatic tissues are largely unknown. Now, researchers at Arizona State University have developed ...

RNA-Seq Blog Transcriptome Research & Industry News

Tag Archives: polyA-tagging

Quartz-Seq2 – a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads

PAT-Seq – polyA-tagging and sequencing for tissue-specific expression analysis changes in an in vivo setting

RNA-Seq of the tumor microenvironment yielded an excellent correlation with immunohistochemistry and flow cytometry data

Single-cell RNA sequencing reveals how aggressive T cells can make heart disease even worse

Sequencing of archival flu virus RNA from European museum samples provides new insights into 1918 pandemic

Imperial College London partners with laboratory in DR Congo for rapid polio detection via NGS of viral RNA

How machine learning and RNA-seq are helping patients diagnosed with the most common childhood cancer

nf-rnaSeqCount – a Nextflow pipeline for obtaining raw read counts from RNA-seq data

Characterization and sequence mapping of large RNA and mRNA therapeutics using mass spectrometry