Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally disregarded, while alternative polyadenylation artifacts are attributed to internal priming. Researchers ...
Read More »TED-Seq identifies the dynamics of Poly(A) length during ER stress
Post-transcriptional RNA processing is a core mechanism of gene expression control in cell stress response. The poly(A) tail influences mRNA translation and stability, but it is unclear whether...
Read More »RNA-Seq reveals widespread intronic polyadenylation diversifies immune cell transcriptomes
Alternative cleavage and polyadenylation (ApA) is known to alter untranslated region (3’UTR) length but can also recognize intronic polyadenylation (IpA) signals to generate transcripts that lose part or all of the coding region. Researchers from Memorial Sloan Kettering Cancer Center ...
Read More »Genome-wide profiling of the 3′ ends of polyadenylated RNAs
Alternative polyadenylation (APA) diversifies the 3′ termini of a majority of mRNAs in most eukaryotes, and is consequently inferred to have substantial consequences for the utilization of post-transcriptional regulatory mechanisms. Since conventional RNA-sequencing methods do not accurately define mRNA termini, ...
Read More »Poly(A)-ClickSeq – click-chemistry for next-generation 3΄-end sequencing without RNA enrichment or fragmentation
The recent emergence of alternative polyadenylation (APA) as an engine driving transcriptomic diversity has stimulated the development of sequencing methodologies designed to assess genome-wide polyadenylation events. The goal of these approaches is to enrich, partition, capture and ultimately sequence poly(A) ...
Read More »RPAD – High-purity isolation of circular RNA
High-throughput RNA sequencing methods coupled with specialized bioinformatic analyses have recently uncovered tens of thousands of unique circular (circ)RNAs, but their complete sequences, genes of origin and functions are largely unknown. Given that circRNAs lack free ends and are thus ...
Read More »RNA-Seq sheds new light on the broad spectrum of action of polyadenylation on gene expression
Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. Researchers at the CNRS used a combination of 5΄-tagRACE and RNA-seq to analyze the total RNA content from ...
Read More »PAT-seq – genome wide 3′-UTR dynamics
A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, researchers at Monash University developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. ...
Read More »APADB: a database for alternative polyadenylation and microRNA regulation events
Alternative polyadenylation (APA) is a widespread mechanism that contributes to the sophisticated dynamics of gene regulation. Approximately 50% of all protein-coding human genes harbor multiple polyadenylation (PA) sites; their selective and combinatorial use gives rise to transcript variants with differing ...
Read More »SLaP Mapper: a webserver for identifying and quantifying spliced-leader addition and polyadenylation site usage
The Kinetoplastida are a diverse and globally distributed class of free-living and parasitic single-celled eukaryotes that collectively cause a significant burden on human health and welfare. In kinetoplastids individual genes do not have promoters, but rather all genes are arranged ...
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