RNA-Seq sheds new light on the broad spectrum of action of polyadenylation on gene expression

April 25, 2017 Leave a comment 3,229 Views

Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. Researchers at the CNRS used a combination of 5΄-tagRACE and RNA-seq to analyze the total RNA content from ...

Polymerase processivity is the mechanistic origin of coverage biases during scRNA-Seq library preparation

November 16, 2016 Leave a comment 3,360 Views

Experimental procedures for preparing RNA-seq and single-cell (sc) RNA-seq libraries are based on assumptions regarding their underlying enzymatic reactions. Here, researchers at the University of Warwick show that the fairness of these assumptions varies within libraries: coverage by sequencing reads ...

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Tag Archives: polymerase

RNA-Seq sheds new light on the broad spectrum of action of polyadenylation on gene expression

Polymerase processivity is the mechanistic origin of coverage biases during scRNA-Seq library preparation

Establishment of a hybridoma sequencing pipeline for sequencing of antibody variable regions

RNA-seq identifies stochastic RNA editing of synaptic genes in single neurons

NVIGEN pioneers breakthrough in high-resolution tissue RNA sequencing with magnetic nanoparticle capture

RNA folding studies by tRNA structure-seq inside peptide-rich droplets reveal roles of modified nucleosides at the origin of life

GiRAFR – gRNA detection and annotation in single-cell CRISPR screens

RNA sequencing reaveals novel intracellular signaling mechanisms that promote melanoma growth

AAV-Perturb-seq – adeno-associated virus (AAV)-mediated direct in vivo single-cell CRISPR screening