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Tag Archives: Preimplantation

Rainbow-Seq – combining cell lineage tracing with single-cell RNA sequencing in preimplantation embryos

October 2, 2018 Leave a comment 2,033 Views

rna-seq

Researchers at UCSD have developed the Rainbow-seq technology to trace cell division history and reveal single-cell transcriptomes. With distinct fluorescent protein genes as lineage markers...

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Akebia saponin D ameliorates metabolic syndrome (MetS) via remodeling gut microbiota and attenuating intestinal barrier injury
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Rubisco activase A (RcaA) is a central node in overlapping gene network of drought and salinity in Barley (Hordeum vulgare L.) and may contribute to combined stress tolerance
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The Arabidopsis STE20/Hippo kinase SIK1 regulates polarity independently of PIN proteins
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Nitrate dose-responsive transcriptome analysis identifies transcription factors and small secreted peptides involved in nitrogen response in Tartary buckwheat
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Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization
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Evolution of Resistance to Phenazine Antibiotics in <em>Staphylococcus aureus</em> and Its Role During Coinfection with <em>Pseudomonas aeruginosa</em>
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

    6 hours ago
  • rna-seq

    Uncovering the Anti-Myeloma Resistance Files

    7 hours ago
  • Long term and short-term effects of RNA fixation on Single Cell RNA Sequencing

    24 hours ago
  • rna-seq

    Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source

    1 day ago
  • rna-seq

    2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

    1 day ago
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    scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases

    2 days ago
  • rna-seq

    DEBKS – a tool to detect differentially expressed circular RNA

    2 days ago

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