Tag Archives: protocol

The transcriptome is composed of different types of RNA molecules including mRNAs, tRNAs, rRNAs, and other noncoding RNAs that are found inside a cell at a given time. Analyzing transcriptome patterns can shed light on the functional state of the ...

A team led by researchers at the University of Florida present a single-cell RNA amplification approach combining exponential and linear amplification steps using a limited number of PCR cycles and T7-driven in vitro transcription (IVT). Thier approach allows for unlimited ...

Despite the ecological importance of copepods, few Next Generation Sequencing studies (NGS) have been performed on small crustaceans, and a standard method for RNA extraction is lacking. In this study, researchers at the Anton Dohrn Zoological Station, Italy compared three ...

from Genetic Engineering News RNA sequencing (RNA-seq), a means of depicting the transcriptome, is being used with increasing frequency to characterize a growing array of conditions—everything from prenatal birth defects to disorders of the elderly. Yet the technique, which is ...

The inference of gene co-expression networks is a valuable resource for novel hypotheses in experimental research. Routine high-throughput microarray transcript profiling experiments and the rapid development of next-generation sequencing (NGS) technologies generate a large amount of publicly available data, enabling ...