Tag Archives: qpcr

Join the 9th Gene Quantification Event #GQ2019 qPCR dPCR & NGS 2019 Symposium & Industrial Exhibition & Application Workshops main.qPCR-dPCR-NGS-2019.net Main topics: Next Generation Biomarkers — Liquid Biopsy, Multi-Omics, MicroGenomics 18-22 March 2019, in Freising-Weihenstephan, School of Life Sciences, Technical ...

Identification of appropriate reference genes (RGs) is critical to accurate data interpretation in quantitative real-time PCR (qPCR) experiments. In this study, researchers from the University of Queensland have utilised next generation RNA sequencing (RNA-seq) to analyse the transcriptome of a ...

Despite RNA-Seq being the capstone technology for gene expression profiling, qPCR remains the go-to technique for validation. To this day, qPCR validation of microarray data is commonplace, and many see it as necessary, given that microarrays involve hybridization to a glass ...

RNA-sequencing has become the gold standard for whole-transcriptome gene expression quantification. Multiple algorithms have been developed to derive gene counts from sequencing reads. While a number of benchmarking studies have been conducted, the question remains how individual methods perform at ...

While qPCR is widely recognized as among the most accurate of methods for quantifying gene expression, it is highly dependent on the use of reliable, stably expressed reference genes. With the increased availability of high-throughput methods for measuring gene expression, ...

Gene expression measurements are typically performed on a fixed-weight aliquot of RNA, which assumes that the total number of transcripts per cell stays nearly constant across all conditions. In cases where this assumption does not hold (e.g., when comparing cell ...

qPCR dPCR & NGS 2017 Liquid Biopsy, Integrative Big Data Analysis, Biomarker Signature … and beyond Symposium  &  Industrial Exhibition  &  Application Workshops 3 – 7 April 2017,   in Freising-Weihenstephan, School of Life Sciences, Technical University of Munich, Weihenstephan, ...

Whole Genome Profiling Using Small RNA Sequencing, Followed by qPCR Based Validation 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST, May 20th 2015 Click here to check the time of the webinar in your country MicroRNAs (miRNAs) ...

Traditionally, gene expression measurements were performed on “bulk” samples containing populations of thousands of cells. Recent advances in genomic technology have made it possible to measure gene expression in hundreds of individual cells at a time. As a result, cellular ...

  Session 3b: Microgenomics and microRNA and NGS Exosome isolation and holistic expression profiling using RNA-Seq and RT-qPCR Michael W. Pfaffl, Technical University of Münich, Germany