Tag Archives: qpcr

Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation

rna-seq

The collborators at the SEQC/MAQC-III Consortium present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference ...

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Cost comparison for qRT-PCR and RNA-Seq

rna-seq

Gene expression studies are widely diffused in life science research. So far, the most popular techniques adopted for transcript quantification are Northern blot, reverse transcription polymerase chain reaction (RT-PCR), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), microarrays, and RNA ...

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