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Tag Archives: raw reads

RNASeqBrowser – A genome browser for simultaneous visualization of raw strand specific RNA-Seq reads and UCSC genome browser custom tracks

March 3, 2015 Leave a comment 10,030 Views

rna-seq

Strand specific RNAseq data is now more common in RNAseq projects. Visualizing RNAseq data has become an important matter in Analysis of sequencing data. The most widely used visualization tool is the UCSC genome browser that introduced the custom track ...

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Recent RNA-SEQ Pubs

Genome-wide identification and functional characterization of natural antisense transcripts in Salvia miltiorrhiza
27 February 2021
Frequent amplification of HDAC genes and efficacy of HDAC inhibitor chidamide and PD-1 blockade combination in soft tissue sarcoma
27 February 2021
Human airway mast cells proliferate and acquire distinct inflammation-driven phenotypes during type 2 inflammation
27 February 2021
Tumor-specific cytolytic CD4 T cells mediate immunity against human cancer
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RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent
27 February 2021
Insights Gained from Single-Cell Analysis of Immune Cells in the Tumor Microenvironment
27 February 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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  • rna-seq

    Single-cell RNA and protein expression in relation to cell cycle progression

    2 days ago
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    Evaluating capture sequence performance for single-cell CRISPR activation experiments

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    Yale neurologists use RNA sequencing to identify consistent neuroinflammatory response in ICH patients

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