RNA-Seq Blog Transcriptome Research & Industry News
by Peter N. Robinson Institut fur Medizinische Genetik und Humangenetik Charite Universitatsmedizin Berlin
Read More »RNA-Seq Read Mapping and Quantification
Application of RNA-seq to the comprehensive analysis of plant mitochondrial transcriptomes
Researchers at the Academy of Sciences of the Czech Republic propose ways to improve read mapping accuracy and sensitivity such as modifying a reference genome at RNA editing sites, using splicing- and ambiguity-competent aligners, and masking chloroplast- or nucleus-derived sequences. ...
Read More »Accuracy of MicroRNA Discovery Pipelines in Non-Model Organisms Using Closely Related Species Genomes
Mapping small reads to genome reference is an essential and more common approach to identify microRNAs (miRNAs) in an organism. Using closely related species genomes as proxy references can facilitate miRNA expression studies in non-model species that their genomes are ...
Read More »Benchmarks of the read aligner segemehl in comparison with other state-of-the-art methods
Next generation sequencing has become an important tool in molecular biology. Various protocols to investigate genomic, transcriptomic, and epigenomic features across virtually all species and tissues have been devised. For most of these experiments, one of the first crucial steps ...
Read More »Mapping RNA-seq reads visualized
Mapping RNA-seq reads visualized
Read More »Reducing bias in RNA sequencing data: a novel approach to compute counts
In the last decade, Next-Generation Sequencing technologies have been extensively applied to quantitative transcriptomics, making RNA sequencing a valuable alternative to microarrays for measuring and comparing gene transcription levels. Although several methods have been proposed to provide an unbiased estimate ...
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