Tag Archives: reference genes

Identification of appropriate reference genes (RGs) is critical to accurate data interpretation in quantitative real-time PCR (qPCR) experiments. In this study, researchers from the University of Queensland have utilised next generation RNA sequencing (RNA-seq) to analyse the transcriptome of a ...

While qPCR is widely recognized as among the most accurate of methods for quantifying gene expression, it is highly dependent on the use of reliable, stably expressed reference genes. With the increased availability of high-throughput methods for measuring gene expression, ...