RNA-Sequencing (RNA-Seq) has become a widely used approach to study quantitative and qualitative aspects of transcriptome data. The variety of RNA-Seq protocols, experimental study designs and the characteristic properties of the organisms under investigation greatly affect downstream and comparative analyses. ...
Read More »Finding RNA Thermometers with Next-Gen Sequencing
Temperature is one of the decisive signals that a mammalian pathogen has entered its warm-blooded host. Among the many ways to register temperature changes, bacteria often use temperature-modulated structures in the untranslated region of mRNAs. In this article, the authors ...
Read More »TargetRNA2 – identifying targets of small regulatory RNAs in bacteria
Many small, noncoding RNAs (sRNAs) in bacteria act as posttranscriptional regulators of messenger RNAs. TargetRNA2 is a web server that identifies mRNA targets of sRNA regulatory action in bacteria. As input, TargetRNA2 takes the sequence of an sRNA and the ...
Read More »Penn biologists establish new method for studying RNA’s regulatory ‘footprint’
Increasingly, biologists have come to realize that RNA is not merely a transitional state between DNA and proteins but plays a major role in determining whether and how genes are turned into a protein product. Gaining a deeper understanding of ...
Read More »