RNA-Sequencing (RNA-seq) is now commonly used to reveal quantitative spatiotemporal snapshots of the transcriptome, the structures of transcripts (splice variants and fusions) and landscapes of expressed mutations. However, standard approaches for library construction typically require relatively high amounts of input ...Read More »
Polymerase processivity is the mechanistic origin of coverage biases during scRNA-Seq library preparation
Experimental procedures for preparing RNA-seq and single-cell (sc) RNA-seq libraries are based on assumptions regarding their underlying enzymatic reactions. Here, researchers at the University of Warwick show that the fairness of these assumptions varies within libraries: coverage by sequencing reads ...Read More »
By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.
from Nature Methods by Jeremy E Wilusz The use of high-throughput RNA sequencing (RNA-seq) has revealed an extraordinarily diverse population of functional RNAs present in cells. However, current approaches poorly detect RNAs that are highly modified and/or extensively folded, such ...Read More »