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Tag Archives: Ribo-ODDR

Ribo-ODDR – Oligo design pipeline for experiment-specific rRNA depletion in ribo-seq

March 19, 2021 Leave a comment 547 Views

rna-seq

Ribosome Profiling (Ribo-seq) has revolutionized the study of RNA translation by providing information on ribosome positions across all translated RNAs with...

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Recent RNA-SEQ Pubs

LRRc17 controls BMSC senescence via mitophagy and inhibits the therapeutic effect of BMSCs on ovariectomy-induced bone loss
17 April 2021
A transcriptional regulatory module controls lipid accumulation in soybean
17 April 2021
Overlapping and stress-specific transcriptomic and hormonal responses to flooding and drought in soybean
17 April 2021
RNA-Seq Transcriptome Analysis of the Liver and Brain of the Black Carp (Mylopharyngodon piceus) During Fasting
17 April 2021
Author Correction to: Single-cell RNA-seq reveals a concomitant delay in differentiation and cell cycle of aged hematopoietic stem cells
17 April 2021
Bioinformatics and survival analysis of glia maturation factor-γ in pan-cancers
17 April 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    scGNN – a novel graph neural network framework for single-cell RNA-Seq analyses

    2 days ago
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