The measurement of RNA abundance derived from massively parallel sequencing experiments is an essential technique. Methods that reduce ribosomal RNA levels are usually required prior to sequencing library construction because ribosomal RNA typically...
Read More »Ribo-depletion in RNA-Seq – Which ribosomal RNA depletion method works best?
Summary: This blogpost is focussed on ribosomal RNA (rRNA) depletion methods frequently applied to improve and economize RNA-Seq experiments. The Rise of RNA-Seq RNA-Seq Overtakes Microarrays The use of Next-Generation RNA Sequencing (RNA-Seq) has recently overtaken that of DNA-based microarrays to ...
Read More »New England Biolabs® Introduces New Ribosomal RNA Depletion Technology from Genomic Health .
IPSWICH, Mass., Aug. 19, 2014 /PRNewswire/ — New England Biolabs, Inc. (NEB®) today announced that NEB has exclusively in-licensed rights from Genomic Health, Inc. to its technology for the depletion of abundant RNAs, which is instrumental to performing sensitive RNA-seq experiments ...
Read More »Comparison of RNA-Seq by poly (A) capture, ribosomal RNA depletion, and DNA microarray for expression profiling
RNA sequencing (RNA-Seq) is often used for transcriptome profiling as well as the identification of novel transcripts and alternative splicing events. Typically, RNA-Seq libraries are prepared from total RNA using poly(A) enrichment of the mRNA (mRNA-Seq) to remove ribosomal RNA ...
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