Tag Archives: Ribosomal RNA

The measurement of RNA abundance derived from massively parallel sequencing experiments is an essential technique. Methods that reduce ribosomal RNA levels are usually required prior to sequencing library construction because ribosomal RNA typically...

A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative non-ribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oligo(dT)-priming of messenger RNAs to exclude ribosomal...

Peripheral blood is a highly accessible biofluid providing a rich source of information about human physiology and health status. However, for studies of the blood transcriptome with RNA sequencing (RNA-Seq) techniques, high levels of hemoglobin mRNAs...

Global transcriptomic analysis via RNA-seq is often hampered by the high abundance of ribosomal (r)RNA in bacterial cells. To remove rRNA and enrich coding sequences, subtractive hybridization procedures have become the approach of choice prior to RNA-seq, with their efficiency ...

Researchers at the Broad Institute have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. This method uses targeted RNase H-based digestion ...

RNAcentral, the first unified resource for all types of non-coding RNA data, has been launched today by the RNAcentral Consortium. It aggregates information from a federation of expert databases, and provides tools for easy browsing. The initial release of RNAcentral ...

IPSWICH, Mass., Aug. 19, 2014 /PRNewswire/ — New England Biolabs, Inc. (NEB®) today announced that NEB has exclusively in-licensed rights from Genomic Health, Inc. to its technology for the depletion of abundant RNAs, which is instrumental to performing sensitive RNA-seq experiments ...

by Natalie McDonald, PhD RNA in the cell is comprised of a variety of populations, including traditional poly-adenylated messenger RNA (polyA RNA), non-adenlyated RNA, which may be coding or regulatory, ribosomal RNA (rRNA) and small and micro RNA.  The first ...

RNA sequencing (RNA-Seq) is often used for transcriptome profiling as well as the identification of novel transcripts and alternative splicing events. Typically, RNA-Seq libraries are prepared from total RNA using poly(A) enrichment of the mRNA (mRNA-Seq) to remove ribosomal RNA ...

A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut ...