Investigating CRISPR RNA Biogenesis and Function Using RNA-Seq

May 20, 2015 Leave a comment 6,016 Views

The development of deep sequencing technology has greatly facilitated transcriptome analyses of both prokaryotes and eukaryotes. RNA-sequencing (RNA-seq), which is based on massively parallel sequencing of cDNAs, has been used to annotate transcript boundaries and revealed widespread antisense transcription as ...

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Tag Archives: RNA Biogenesis

Investigating CRISPR RNA Biogenesis and Function Using RNA-Seq

GiRAFR – gRNA detection and annotation in single-cell CRISPR screens

RNA sequencing reaveals novel intracellular signaling mechanisms that promote melanoma growth

AAV-Perturb-seq – adeno-associated virus (AAV)-mediated direct in vivo single-cell CRISPR screening

capTEs – Cas9-assisted profiling transposable elements expression sequencing

eSPET-seq – genome-wide probing of eukaryotic nascent RNA structure

DISCERN – deep single-cell expression reconstruction for improved cell clustering and cell subtype and state detection

Advancements in genome sequencing propel global RNA sequencing market growth to 2030