Tag Archives: RNA Extraction

A method for extracting high-quality RNA from diverse plants for next-generation sequencing and gene expression analyses

To study expression in plants, high-quality RNA must be extracted in quantities sufficient for subsequent cDNA library construction. Field-based collections are often limited in quantity and quality of tissue and are typically preserved in RNAlater. Obtaining sufficient and high-quality yield ...

Read More »

Influence of RNA extraction methods and library selection schemes on RNA-seq data

rna-seq

Gene expression analysis by RNA sequencing is now widely used in a number of applications surveying the whole transcriptomes of cells and tissues. The recent introduction of ribosomal RNA depletion protocols, such as RiboZero, has extended the view of the ...

Read More »

Success in RNA-Seq – Sample Prep and Workflow considerations

rna-seq

by Natalie McDonald, PhD RNA in the cell is comprised of a variety of populations, including traditional poly-adenylated messenger RNA (polyA RNA), non-adenlyated RNA, which may be coding or regulatory, ribosomal RNA (rRNA) and small and micro RNA.  The first ...

Read More »

RNA-Seq Blog – Poll Results

rna-seq

We asked: Which step of an RNA-Seq experiment is the greatest source of technical variability? Thanks to everyone who participated! Check out the new poll question in the narrow sidebar and cast your vote.

Read More »

Norgen Biotek

Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to providing dedicated and expert support services to our customers and partners worldwide. http://norgenbiotek.com/product-category-list-rna-purification-kits.php

Read More »