By the time you reach the end of this sentence, RNA folding will have taken place in your body more than 10 quadrillion times. The folding of RNA is essential to life, yet because it happens so rapidly, researchers have ...
Read More »rG4-seq – a new method for RNA G-quadruplex (rG4) transcriptome profiling
Researchers from the University of Cambridge introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, the researchers generated a global in vitro ...
Read More »SPARSE: Quadratic Time Simultaneous Alignment and Folding of RNAs Without Sequence-Based Heuristics
RNA-Seq experiments have revealed a multitude of novel ncRNAs. The gold standard for their analysis based on simultaneous alignment and folding (Sankoff, 1985) suffers from extreme time complexity of O(n6). Subsequently, numerous faster “Sankoff-style” approaches have been suggested. Commonly, the ...
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