In eukaryotic cells, alternative cleavage of 3′ untranslated regions (UTRs) can affect transcript stability, transport and translation. For polyadenylated (poly(A)) transcripts, cleavage sites can be characterized with short-read sequencing using specialized library construction methods. However, for large-scale cohort studies as ...
Read More »Challenges and strategies for RNA-Seq data analysis
RNA-seq is a methodology for RNA profiling based on next-generation sequencing that enables to measure and compare gene expression patterns at unprecedented resolution. Although the appealing features of this technique have promoted its application to a wide panel of transcriptomics ...
Read More »RAP: RNA-seq Pipeline web tool – tutorial 1: analysis submission and monitoring
RAP: RNA-Seq Analysis Pipeline RNA-Seq technology is becoming widely used in various transcriptomics studies; however, analyzing and interpreting the RNA-Seq data face serious challenges due to transcriptome complexity. A complete RNA-seq analysis involves several steps and the data can be ...
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