Although RNA-Seq data provide unprecedented isoform-level expression information, detection of alternative isoform regulation (AIR) remains difficult, particularly when working with an incomplete transcript annotation. Researchers at the National Human Genome Research Institute introduce JunctionSeq, a new method that builds on the ...
Read More »SplAdder – Identification, quantification and testing of alternative splicing events from RNA-Seq data
Understanding the occurrence and regulation of alternative splicing (AS) is a key task towards explaining the regulatory processes that shape the complex transcriptomes of higher eukaryotes. With the advent of high-throughput sequencing of RNA (RNA-Seq), the diversity of AS transcripts ...
Read More »Penn Researchers Illuminate “Dark Side” of the Transcriptome
New approach to quantifying gene “read-outs” reveals important variations in protein synthesis and has implications for understanding neurodegenerative diseases PHILADELPHIA— A new way of mapping the “transcriptome” — the collection of RNA read-outs that are expressed by a cell’s active ...
Read More »Featured RNA-Seq Job – Research Associate – Next-generation RNA Sequencing
Hiring department – Icmb-PI-Lambowitz Monthly salary – $3,750+ depending on qualifications Hours per week – 40.00 Standard from 800AM to 500PM Posting number – 15-05-07-01-0708 Job Status – Open FLSA status – Exempt Earliest Start Date – Immediately Position Duration ...
Read More »MIRALCS – microwell full-length mRNA amplification and library construction from a single-cell
Viral infection causes multiple forms of human cancer, and HPV infection is the primary factor in cervical carcinomas. Recent single-cell RNA-seq studies highlight the tumor heterogeneity present in most cancers, but virally induced tumors have not been studied. HeLa is ...
Read More »Research Fellow Position Available – RNA Splicing
Requisition Number 15-0350 Post Date 8/11/2015 Title RESEARCH FELLOW Department Ocular Genomics-Pierce Shift Day Hours Per Week 40 City Boston State MA Description The Ocular Genomics Institute at the Massachusetts Eye and Ear, Harvard Medical School is seeking a talented ...
Read More »Most cancer transcriptomes are bloated by widespread intron retention
by JM Kocarnik at FHCRC Alternative splicing of specific genes can contribute to cancer initiation, progression, and metastasis. With the advent of cancer genome sequencing, it was discovered that many tumor types contain high frequencies of somatic mutations affecting components ...
Read More »Introduction to RNA Sequencing
This lecture explains about the RNA sequencing process and the methods or RNA splicing is explained. It also states the use of RNA sequencing in molecular biology. RNA Sequencing, often known as entire Transcriptome Shotgun Sequencing, is a science that ...
Read More »A comparative study of RNA-seq analysis strategies
Three principal approaches have been proposed for inferring the set of transcripts expressed in RNA samples using RNA-seq. The simplest approach uses curated annotations, which assumes the transcripts in a sample are a subset of the transcripts listed in a ...
Read More »Developmental Analysis of Spliceosomal snRNA Isoform Expression with RNA-Seq
Pre-mRNA splicing is a critical step in eukaryotic gene expression that contributes to proteomic, cellular and developmental complexity. Small nuclear (sn)RNAs are core spliceosomal components, however, the extent to which differential expression of snRNA isoforms regulates splicing is completely unknown. ...
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