RNA-Seq Blog Transcriptome Research & Industry News
Crops often have multiple genomes hybridised from their parents, a condition known as polyploidy. Polyploidy is a major route to the formation of new...
Read More »RNA SHAPE-Structure-seq offers new perspective for breeding polyploid crops
PORE-cupine – determination of isoform-specific RNA structure with nanopore long reads
Current methods for determining RNA structure with short-read sequencing cannot capture most differences between distinct transcript isoforms. Researchers at A*STAR, Singapore present RNA structure analysis using nanopore sequencing...
Read More »Sequencing and structure probing of long RNAs using MarathonRT
Reverse transcriptase (RT) enzymes are indispensable tools for interrogating diverse aspects of RNA metabolism and transcriptome composition. Due to the growing interest in sequence and...
Read More »Viral RNA structure analysis using DMS-MaPseq
RNA structure is critically important to RNA viruses in every part of the replication cycle. RNA structure is also utilized by DNA viruses in order to regulate gene expression and interact with host factors...
Read More »StructRNAfinder – an automated pipeline and web server for RNA families prediction
The function of many noncoding RNAs (ncRNAs) depend upon their secondary structures. Over the last decades, several methodologies have been...
Read More »Summer School on RNA-protein interactions RNA Structure and Biology workshop
October 2–6 2017 CEITEC MU, University Campus Bohunice Kamenice 5, Brno, Czech Republic The objective of the summer school is to provide participants with an overview on the most up-to-date topics in RNA research. The lectures from leading experts in ...
Read More »RNA Structure Framework – automatic transcriptome-scale reconstruction of RNA structures from probing data
The rapidly increasing number of discovered non-coding RNAs makes the understanding of their structure a key feature toward a deeper comprehension of gene expression regulation. Various enzymatic- and chemically- based approaches have been recently developed to allow whole-genome studies of ...
Read More »Probing RNA Structure in Cells Using Targeted Structure-Seq
Models for how lncRNAs function depend on thermodynamically stable secondary and higher-order structures that RNAs can form in the context of a cell. Probing accessible RNA bases can provide data to build models of RNA conformation that provide insight into ...
Read More »RNA-Seq workflow for comprehensive profiling of reverse transcription encounters with m1A
The combination of Reverse Transcription (RT) and high-throughput sequencing has emerged as a powerful combination to detect modified nucleotides in RNA via analysis of either abortive RT-products or of the incorporation of mismatched dNTPs into cDNA. Here researchers from the ...
Read More »RNA structure from deep sequencing
from Nature Biotechnology by Eric Westhof Solving the three-dimensional structure of an RNA molecule means laborious study by X-ray crystallography or nuclear magnetic resonance spectroscopy. However, faster complementary methods are on the horizon, many involving deep sequencing and sophisticated computational ...
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