The astounding regenerative abilities of planarian flatworms prompt steadily growing interest in examining their molecular foundation. Planarian regeneration was found to require hundreds of genes and is hence a complex process. Thus, RNA...
Read More »Host-Pathogen Transcriptomics by Dual RNA-Seq
Transcriptomics, i.e., the quantification of cellular RNA transcripts, is a powerful way to gauge the physiological state of either bacterial or eukaryotic cells under a given condition. However, traditional...
Read More »A method to compare RNA-Seq datasets from different library types
The availability of fast alignment-free algorithms has greatly reduced the computational burden of RNA-seq processing, especially for relatively poorly assembled genomes. Using these approaches, previous RNA-seq datasets could potentially be processed and integrated with newly sequenced libraries. Confounding factors in ...
Read More »Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes
Global transcriptomic analysis via RNA-seq is often hampered by the high abundance of ribosomal (r)RNA in bacterial cells. To remove rRNA and enrich coding sequences, subtractive hybridization procedures have become the approach of choice prior to RNA-seq, with their efficiency ...
Read More »RiboCop rRNA Depletion Kit enables removal of ribosomal RNA (rRNA) from human total RNA
Lexogen’s RiboCop rRNA Depletion Kit enables removal of ribosomal RNA (rRNA) from human total RNA and is suited for Next Generation Sequencing (NGS) applications. Samples are treated using a set of affinity probes for specific depletion of rRNA sequences. Number ...
Read More »Detection of Plant Viruses in Natural Environments by Using RNA-Seq
Sequencing of RNA by next generation sequencers, RNA-Seq, is revolutionizing virus detection. In addition to the unbiased detection of various viruses from wild plants in natural environments, RNA-Seq also allows for the parallel collection of host plant transcriptome data. Host ...
Read More »New England Biolabs® Introduces New Ribosomal RNA Depletion Technology from Genomic Health .
IPSWICH, Mass., Aug. 19, 2014 /PRNewswire/ — New England Biolabs, Inc. (NEB®) today announced that NEB has exclusively in-licensed rights from Genomic Health, Inc. to its technology for the depletion of abundant RNAs, which is instrumental to performing sensitive RNA-seq experiments ...
Read More »Influence of RNA extraction methods and library selection schemes on RNA-seq data
Gene expression analysis by RNA sequencing is now widely used in a number of applications surveying the whole transcriptomes of cells and tissues. The recent introduction of ribosomal RNA depletion protocols, such as RiboZero, has extended the view of the ...
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