Circular RNAs (circRNAs) are a class of non-coding RNAs that are widely expressed in various cell lines and tissues of many organisms. Although the exact function of many circRNAs is largely unknown, the cell type- and tissue-specific circRNA expression has ...
Read More »Performance of RNA-seq quantification pipelines is “generally poor”
Obtaining RNA-seq measurements involves a complex data analytical process with a large number of competing algorithms as options. There is much debate about which of these methods provides the best approach. Unfortunately, it is currently difficult to evaluate their performance ...
Read More »Tutorial – RNA-seq differential expression & pathway analysis with Sailfish, DESeq2, GAGE, and Pathview
Comparing the normalization methods for the differential analysis of Illumina high-throughput RNA-Seq data
Recently, rapid improvements in technology and decrease in sequencing costs have made RNA-Seq a widely used technique to quantify gene expression levels. Various normalization approaches have been proposed, owing to the importance of normalization in the analysis of RNA-Seq data. ...
Read More »RNA-Skim – a rapid method for RNA-Seq quantification at transcript level
RNA-Seq technique has been demonstrated as a revolutionary means for exploring transcriptome because it provides deep coverage and base pair-level resolution. RNA-Seq quantification is proven to be an efficient alternative to Microarray technique in gene expression study, and it is ...
Read More »Computational Method Dramatically Speeds Up Estimates of Gene Expression, CMU, UMD Researchers Report
“Sailfish” Method Could Pay Dividends as Genomic Medicine Expands Contact: Byron Spice / 412-268-9068 / bspice@cs.cmu.edu PITTSBURGH—With gene expression analysis growing in importance for both basic researchers and medical practitioners, researchers at Carnegie Mellon University and the University of Maryland ...
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