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Tag Archives: sample indexing

DeTCT – a purely quantitative digital gene expression sample processing and analysis package

August 7, 2015 Leave a comment 4,820 Views

rna-seq

Many RNA-seq methods are excellent tools for in-depth mRNA expression analysis of small numbers of samples and provide information on the entire RNA molecule, alternative splicing and the quantity of transcript. However, they require an involved library preparation and often ...

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Schistosoma mansoni alter transcription of immunomodulatory gene products following in vivo praziquantel exposure
3 March 2021
Establishment of a Cuscuta campestris-mediated enrichment system for genomic and transcriptomic analyses of 'Candidatus Liberibacter asiaticus'
3 March 2021
The transcriptome characteritics of vestibular organs from delayed endolymphatic hydrops patients(Menieres disease)
3 March 2021
Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis
3 March 2021
Transcriptomic and physiological responses of the tropical reef calcified macroalga Amphiroa fragilissima to elevated temperature
3 March 2021
The type 2 asthma mediator IL-13 inhibits SARS-CoV-2 infection of bronchial epithelium
3 March 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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  • rna-seq

    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    11 hours ago
  • rna-seq

    RNA sequencing brings hope for endangered wild cats

    12 hours ago
  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

    1 day ago
  • rna-seq

    Uncovering the Anti-Myeloma Resistance Files

    1 day ago
  • Long term and short-term effects of RNA fixation on Single Cell RNA Sequencing

    2 days ago
  • rna-seq

    Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source

    2 days ago
  • rna-seq

    2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

    2 days ago

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