Tag Archives: sample size

Next generation sequencing provides a count of RNA molecules in the form of short reads, yielding discrete, often highly non-normally distributed gene expression measurements. Although Negative Binomial (NB) regression has been generally accepted in the analysis of RNA sequencing (RNA-Seq) ...

(RNA-seq) has become a routine technique in transcriptome analysis, where identifying differential expression (DE) remains a major task. As expression is a stochastic process in nature, the technological improvement in RNA-seq cannot bypass the presence of biological variability. It has ...

It is crucial for researchers to optimize RNA-seq experimental designs for differential expression detection. Currently, the field lacks general methods to estimate power and sample size for RNA-Seq in complex experimental designs, under the assumption of the negative binomial distribution. ...