Tag Archives: sensitivity

RNA-Seq made possible the global identification of fusion transcripts, i.e. “chimeric RNAs”. Even though various software packages have been developed to serve this purpose, they behave differently in different datasets provided by different developers. It is important for both users, ...

Next generation sequencing has led to diverse RNA-Seq methods, but one drawback is sensitivity. Conventional RNA-Seq requires more than 10 ng of RNA from 50–100 cells. But increasingly researchers are interested in profiling gene expression in single cells. Ramskold and ...