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Tag Archives: Sequencing coverage

Overcome analytical bottlenecks in RNA-Seq experiments with limited number of replicates and low sequencing coverage

January 4, 2018 Leave a comment 3,516 Views

rna-seq

In current statistical methods for calling differentially expressed genes in RNA-Seq experiments, the assumption is that an adjusted observed gene count represents an unknown true gene count...

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Recent RNA-SEQ Pubs

Deep sequencing of the Sanghuangporus vaninii transcriptome reveals dynamic landscapes of candidate genes involved in the biosynthesis of active compounds
1 March 2021
Integrating imaging and RNA-seq improves outcome prediction in cervical cancer
1 March 2021
Gut microbiota shape the inflammatory response in mice with an epithelial defect
1 March 2021
Selection and validation of reference genes for quantitative Real-time PCR analysis in Amana edulis
1 March 2021
Sphingosine-1-phosphate receptor 3 is implicated in BBB injury via the CCL2-CCR2 axis following acute intracerebral hemorrhage
1 March 2021
Molecular characterization of DICER1-mutated pituitary blastoma
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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  • rna-seq

    scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases

    23 hours ago
  • rna-seq

    DEBKS – a tool to detect differentially expressed circular RNA

    24 hours ago
  • rna-seq

    Single-cell RNA and protein expression in relation to cell cycle progression

    4 days ago
  • rna-seq

    Evaluating capture sequence performance for single-cell CRISPR activation experiments

    4 days ago
  • rna-seq

    Seven Bridges Selected by Pfizer to Support Data Management Solution for Single-Cell RNA Sequencing Data

    4 days ago
  • rna-seq

    Single-cell mapper (scMappR) – using scRNA-seq to infer the cell-type specificities of differentially expressed genes

    5 days ago
  • rna-seq

    Featured RNA-Seq Job – Staff Bioinformatics Scientist, RNA-Seq

    5 days ago

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