Tag Archives: sequencing

EMBL-EBI Training | Introduction to RNA-seq and functional interpretation Gain an introduction to the technology, data analysis, tools, and resources used in RNA sequencing and transcriptomics. The content will provide a broad overview of the subject area, and introduce participants ...

Many applications of multiple sequence alignments (MSA) involve working with sequences which are not ideal – they can be incomplete, contain errors or just be highly divergent with many mismatches. This is very common when working with high throughput sequencing ...

The study of small RNAs provides us with a deeper understanding of the complexity of gene regulation within cells. Of the different types of small RNAs, the most important in mammals are miRNA, tRNA fragments and piRNAs. Using small RNA-seq ...

EMBL Course: Shift your DNA and RNA Sequencing Library Preparation into Hyper-Drive Course Overview The goal of this hands-on course is to teach the fundamentals of RNA and DNA library preparation for next generation sequencing applications. RNA depletion and enrichment strategies ...

Simultaneous sequencing of the genome and transcriptome at the single-cell level is a powerful tool for characterizing genomic and transcriptomic variation and revealing correlative relationships...

The recently introduced Kallisto pseudoaligner has radically simplified the quantification of transcripts in RNA-sequencing experiments.  Researchers from the Keck School of Medicine of USC offer cloud-scale RNAseq pipelines Arkas-Quantification, which deploys Kallisto for parallel cloud computations, and Arkas-Analysis, which annotates ...

News Medical Life Sciences by By Catherine Shaffer, M.Sc. – If DNA is the fundamental code of the genome, RNA is the message. Genes are made active through the process of transcribing the code into RNA, and then translating RNA into DNA. This is known as ...

Grafting of cell lines and primary tumours is a crucial step in the drug development process between cell line studies and clinical trials. AstraZeneca researchers have developed disambiguate, a program for computationally separating the sequencing reads of two species derived ...

Next-generation sequencing (NGS) informs many biological questions with unprecedented depth and nucleotide resolution. These assays have created a need for analytical tools that enable users to manipulate data nucleotide-by-nucleotide robustly and easily. Furthermore, because many NGS assays encode information jointly ...

RNA-Seq is the leading technology for analyzing gene expression on a global scale across a broad spectrum of sample types. However, due to chemical modifications by fixation or degradation due to collection methods, samples often contain an abundance of RNA ...