Tag Archives: sequencing

XSAnno – a framework for building ortholog models in cross-species transcriptome comparisons

rna-seq

The accurate characterization of RNA transcripts and expression levels across species is critical for understanding transcriptome evolution. As available RNA-seq data accumulate rapidly, there is a great demand for tools that build gene annotations for cross-species RNA-seq analysis. However, prevailing ...

Read More »

Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation

rna-seq

The collborators at the SEQC/MAQC-III Consortium present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference ...

Read More »

The concordance between RNA-seq and microarray data depends on chemical treatment and transcript abundance

rna-seq

The concordance of RNA-sequencing (RNA-seq) with microarrays for genome-wide analysis of differential gene expression has not been rigorously assessed using a range of chemical treatment conditions. Here a team led by researchers atuse at the US FDA comprehensive study design ...

Read More »