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Tag Archives: sequential imaging

Error-correction strategy allows precise measurement of transcriptome in single cells

April 21, 2015 Leave a comment 3,995 Views

rna-seq

from Phys.org Howard Hughes Medical Institute (HHMI) scientists have devised a method of visualizing RNA molecules inside cells so that the identity, location, and abundance of more than 1,000 different RNA species can be determined at the same time. The ...

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Melatonin promotes potassium deficiency tolerance by regulating HAK1 transporter and its upstream transcription factor NAC71 in wheat
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Learn more about available bioinformatics tools and pipelines to analyze RNA sequencing data

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    Yale researchers identify tumor reactive immune cells with RNA sequencing to help fight against advanced melanoma

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