Tag Archives: shRNA

In the search for novel therapeutic targets, RNA interference screening has become a valuable tool. High-throughput technologies are now broadly accessible but their assay development from baseline...

Primary neuronal cultures are a useful tool for measuring pharmacological- and transgene-regulated gene expression; however, accurate measurements can be confounded by heterogeneous cell types and inconsistent transfection efficiency. Here researchers from the University of Washington describe their adaptation of a ...

Pooled short hairpin RNA sequencing (shRNA-seq) screens are becoming increasingly popular in functional genomics research, and there is a need to establish optimal analysis tools to handle such data. This open-source shRNA processing pipeline in edgeR provides a complete analysis ...

The Staudt laboratory at NCI performs a number of different genomics methodologies to discover oncogenic mechanisms in lymphoma, all of which require high-throughput DNA sequencing. Specifically, the laboratory developed loss-of-function genetic screens to discover new molecular targets in cancer using ...