Prevalent single cell RNA amplification and sequencing chemistries mainly focus on polyadenylated RNAs in eukaryotic cells by using oligo(dT) primers for reverse transcription. Researchers from Peking University have developed a new RNA amplification method, “easier-seq”, to reverse transcribe and amplify ...
Read More »Single-Cell RNA-Seq Reveals How Zika Virus Causes Fetal Brain Damage
Marco Onorati et al. describe the derivation and characterization, including single-cell RNA-seq, of neocortical and spinal cord neuroepithelial stem (NES) cells to model early human neurodevelopment and Zika virus (ZIKV)-related neuropathogenesis. By analyzing human NES cells, organotypic fetal brain slices, ...
Read More »