Tag Archives: Single Molecule

Estonian scientists have announced the invention of a genetic testing technology to analyse the number of clinical biomarkers at the single-molecule level, which enhances the sensitivity of tests in precision medicine and will make them more affordable in future...

RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here Yale University researchers report TimeLapse-seq, which...

Transcriptional mutagenesis (TM) due to misincorporation during RNA transcription can result in mutant RNAs, or epimutations, that generate proteins with altered properties. TM has long been hypothesized to play a role in aging, cancer, and viral and bacterial evolution. However, ...

A series of recent peer-reviewed scientific publications highlights the growing use of Single Molecule, Real-Time (SMRT®) Sequencing to annotate plant and animal genomes. These reports from the genomics community show that PacBio sequencing consistently detects more genes, alternative splicing events, ...

RNA-Seq has provided valuable information about the transcriptomes from homogenized tissues or cultured cells. But as tissues consist of a heterogeneous pool of cells, specific cell-type differences in gene expression are diluted. Moreover, within the same population, individual cells show ...

Sequencing of RNA provides the possibility to study an individual’s transcriptome landscape and determine allelic expression ratios. Single-molecule protocols generate multi-kilobase reads longer than most transcripts allowing sequencing of complete haplotype isoforms. This allows partitioning the reads into two parental ...

Improvements in maize breeding are required to feed a growing population and will depend on a more complete understanding of global gene expression in the plant. In a new publication from Cold Spring Harbor Laboratory (CSHL), scientists produced the largest ...

Transfer RNAs (tRNA) are the most common RNA molecules in cells and have critical roles as both translators of the genetic code and regulators of protein synthesis. As such, numerous methods have focused on studying tRNA abundance and regulation, with ...

University of Michigan researchers have developed a technique that allows them to efficiently identify snippets of genetic information called microRNAs in blood. The advance could one day lead to a way to scan for multiple types of cancer at once ...

CAGE (cap analysis gene expression) and RNA-seq are two major technologies used to identify transcript abundances as well as structures. They measure expression by sequencing from either the 5′ end of capped molecules (CAGE) or tags randomly distributed along the ...