Human Lung Cell Atlas launched! On 11 March 2022 the discovAIR consortium officially launched the Human Lung Cell Atlas (HLCA) during the ERS Lung Science Conference in Estoril, Portugal. Malte Lücken (Helmholtz Center Munich) introduced the HLCA during his presentation ...
Read More »Systematic comparison of single-cell and single-nucleus RNA-sequencing methods
The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively...
Read More »Taming the transcriptome with RNA-Seq
There’s a new RNA-seq in town. Although the old RNA-seq administered a certain rough justice, profiling RNA species well enough to support a relatively crude conception of the transcriptome, the new RNA-seq is more refined...
Read More »DroNc-Seq – Single-nucleus RNA sequencing, droplet by droplet
DroNc-Seq — a technology that merges single-nucleus RNA sequencing with microfluidics — brings new scale to gene expression studies in complex tissues. from The Broad Institute by Tom Ulrich – Last year Broad researchers described a single-nucleus RNA sequencing method called sNuc-Seq. This ...
Read More »Div-Seq – A single nucleus RNA-Seq method reveals dynamics of rare adult newborn neurons in the CNS
Transcriptomes of individual neurons provide rich information about cell types and dynamic states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation from dense adult tissue is challenging, and markers for each phase are ...
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