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Tag Archives: small cytoplasmic RNA

Impact of single cell RNA sequencing on kidney organoids research

February 28, 2020 Leave a comment 1,656 Views

rna-seq

Methods to differentiate human pluripotent stem cells into kidney organoids were first introduced about 5 years ago, and since that time, the field...

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Recent RNA-SEQ Pubs

Repeated morphine exposure activates synaptogenesis and other neuroplasticity-related gene networks in the dorsomedial prefrontal cortex of male and female rats
24 February 2021
Disruption of DNA polymerase ζ engages an innate immune response
24 February 2021
Correlation of immune infiltration with clinical outcomes in breast cancer patients: The 25-gene prognostic signatures model
24 February 2021
Transcriptome analysis of yellow passion fruit in response to cucumber mosaic virus infection
24 February 2021
Evaluating Capture Sequence Performance for Single-Cell CRISPR Activation Experiments
24 February 2021
RNA Sequencing of Decidua Reveals Differentially Expressed Genes in Recurrent Pregnancy Loss
24 February 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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  • rna-seq

    Single-cell mapper (scMappR) – using scRNA-seq to infer the cell-type specificities of differentially expressed genes

    7 hours ago
  • rna-seq

    Featured RNA-Seq Job – Staff Bioinformatics Scientist, RNA-Seq

    8 hours ago
  • Introduction to NGS – RNA-Seq Data Analysis – DNA Variant Calling

    1 day ago
  • rna-seq

    Yale neurologists use RNA sequencing to identify consistent neuroinflammatory response in ICH patients

    1 day ago
  • rna-seq

    Resolve Biosciences’ Molecular Cartography platform detects specific gene expression patterns in SARS-CoV-2 not seen with existing single-cell RNA-seq technologies

    1 day ago
  • rna-seq

    Molecular Stethoscope publishes human proof-of-concept study for its cell-free mRNA based NGS liquid biopsy technology platform

    1 day ago
  • rna-seq

    Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip

    2 days ago

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