Tag Archives: small RNA libraries

With the rapid accumulation of publicly available small RNA sequencing datasets, third-party meta-analysis across many datasets is becoming increasingly powerful. Although removing the 3´ adapter is an essential step for small RNA sequencing analysis, the adapter sequence information is not ...

Computational prediction of microRNAs (miRNAs) is one of the most important approaches in microRNA studies. While validation of the predicted microRNAs’ precise sequences is essential for further studies on their biogenesis, evolution, and functions, computational miRNA prediction methods, however, often ...

RNA-related applications of the next-generation sequencing (NGS) technologies require context-specific interpretations: e.g., sequence mismatches may indicate sites of RNA editing, or uneven read coverage often points to mature form of microRNA. Existing visualization tools traditionally show RNA molecules in two ...

MicroRNAs play an important role in regulation and disease progression, and thus are increasingly being characterized by next-generation sequencing (NGS). One of the challenges in deep sequencing of the small RNAs (small RNA-Seq) is in the sample preparation workflow. This ...