RNA-Seq Blog Transcriptome Research & Industry News
The attachment of unique molecular identifiers (UMIs) to RNA molecules prior to PCR amplification and sequencing, makes it possible to amplify...
Read More »Insufficiently complex unique-molecular identifiers (UMIs) distort small RNA sequencing
Comparison of reproducibility, accuracy, sensitivity, and specificity of miRNA quantification platforms
Given the increasing interest in their use as disease biomarkers, the establishment of reproducible, accurate, sensitive, and specific platforms for...
Read More »sRNAPipe – a Galaxy-based pipeline for bioinformatic in-depth exploration of small RNAseq data
The field of small RNA is one of the most investigated research areas since they were shown to regulate transposable elements and gene...
Read More »piRNA analysis from small RNA-Seq data
With the increasing number of studies focusing on PIWI-interacting RNA (piRNAs), it is now pertinent to develop efficient tools dedicated towards piRNA analysis. Researchers at the National Institute of...
Read More »An evaluation of high-throughput isomiR identification tools
MicroRNAs carry out post-transcriptional gene regulation in animals by binding to the 3′ untranslated regions of mRNAs, causing their degradation or translational repression. MicroRNAs influence many biological functions, and dysregulation can therefore disrupt development or even cause death. High-throughput sequencing ...
Read More »RNA-Seq Identifies a Panel of MicroRNAs as Diagnostic Biomarkers for Prostate Cancer
Prostate cancer is the most common non-cutaneous cancer among men; yet, current diagnostic methods are insufficient, and more reliable diagnostic markers need to be developed. One answer that can bridge this gap may lie in microRNAs. These small RNA molecules ...
Read More »DNApi – A De Novo Adapter Prediction Algorithm for Small RNA Sequencing Data
With the rapid accumulation of publicly available small RNA sequencing datasets, third-party meta-analysis across many datasets is becoming increasingly powerful. Although removing the 3´ adapter is an essential step for small RNA sequencing analysis, the adapter sequence information is not ...
Read More »SomaGenics secures $1.8M in funding from NIH to further develop the Real-Seq® technology platform for small RNA sequencing
SomaGenics has been awarded a $1.8M two-year NIH SBIR Phase II grant to further develop its RealSeq®–AC technology for next-generation sequencing (NGS) of small RNAs such as microRNA. Real-Seq®-AC dramatically reduces bias in small RNA sequencing libraries compared to commercially ...
Read More »A workflow for analysis of small RNA sequencing data
Small RNAs (size 20-30 nt) of various types have been actively investigated in recent years, and their subcellular compartmentalization and relative concentrations are likely to be of importance to their cellular and physiological functions. Comprehensive data on this subset of ...
Read More »An evaluation of microRNA alignment techniques
Genomic alignment of small RNA (smRNA) sequences such as microRNAs poses considerable challenges due to their short length (∼21 nucleotides [nt]) as well as the large size and complexity of plant and animal genomes. While several tools have been developed ...
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