Worldwide growth and performance-enhancing substances are used in cattle husbandry to increase productivity. In certain countries however e.g., in the EU, these practices are forbidden to prevent the consumers from potential health risks of substance residues in food. To maximize ...
Read More »Bioo Scientific Granted Patent for Small RNA Sequencing Technology
Bioo Scientific announced today that the United States Patent and Trademark Office (USPTO) has issued U.S. patent US9255291 entitled Oligonucleotide ligation, barcoding and methods and compositions for improving data quality and throughput using massively parallel sequencing. The granted claims describe ...
Read More »Understanding and using small RNA-Seq
David Galas from the Pacific Northwest Diabetes Research Institute presents a seminar on his lab’s efforts, in the context of the Extracellular RNA Communication Consortium (ERCC), to address the problem of sequence-specific bias in small RNA sequencing. This seminar was ...
Read More »Webinar Today – Quantitative MicroRNA Expression Analysis in Tissues and Body Fluids
Whole Genome Profiling Using Small RNA Sequencing, Followed by qPCR Based Validation 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST, May 20th 2015 Click here to check the time of the webinar in your country MicroRNAs (miRNAs) ...
Read More »Bias in Ligation-Based Small RNA Sequencing Library Construction
High-throughput sequencing (HTS) has become a powerful tool for the detection of and sequence characterization of microRNAs (miRNA) and other small RNAs (sRNA). Unfortunately, the use of HTS data to determine the relative quantity of different miRNAs in a sample ...
Read More »Optimization of miRNA-seq data preprocessing
The past two decades of microRNA (miRNA) research has solidified the role of these small non-coding RNAs as key regulators of many biological processes and promising biomarkers for disease. The concurrent development in high-throughput profiling technology has further advanced our ...
Read More »New microRNA Analysis App in BaseSpace
miRNAs are a class of non-coding RNAs mainly involved in post-transcription control of gene expression although they are also involved in other non-canonical functions. Furthermore, they represents an interesting class of biomarkers for a large variety of diseases. Small RNA-sequencing ...
Read More »Oasis – online analysis of small RNA deep sequencing data
Oasis is a web application that allows for the fast and flexible online analysis of small-RNA-seq (sRNA-seq) data. It was designed for the end user in the lab, providing an easy-to-use web frontend including video tutorials, demo data, and best ...
Read More »Reducing Small RNA Sequencing Biases
from Genetic Engineering News by Adam R. Morris & Masoud M. Toloue Randomized Adapter Strategy for Library Preparation Reduces Ligation Bias and Increases Accuracy of Small RNA-Seq The study of small RNAs, including miRNAs, siRNAs, and pi-RNAs, is an ideal ...
Read More »Computational prediction of miRNA genes from small RNA sequencing data
Next-generation sequencing now for the first time allows researchers to gage the depth and variation of entire transcriptomes. However, now as rare transcripts can be detected that are present in cells at single copies, more advanced computational tools are needed ...
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