Tag Archives: smart-seq

This validated protocol for miniaturized cDNA synthesis reactions was developed to take the guesswork out of reaction miniaturization so that you can: Conserve precious sequencing reagents Sequence more single-cells Generate more meaningful data to support your genetic analysis http://info.formulatrix.com/formulatrix-and-takara-bio-present-a-validated-sop-for-quarter-volume-smart-seq-v4-reactions

Gene expression heterogeneity in the pluripotent state of mouse embryonic stem cells (mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and lineage commitment have not been studied systematically at the single-cell level. Here a team led by researchers ...

Single-cell transcriptomics is a transformative method with tremendous potential to illuminate the complexities of gene regulation. Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. The CEL-Seq method is the first method to use in vitro transcription (IVT) ...

Single-cell mRNA sequencing (scRNA-seq) allows to profile heterogeneous cell populations, offering exciting possibilities to tackle a variety of biological and medical questions. A range of methods has been recently developed, making it necessary to systematically compare their sensitivity, accuracy, precision ...

Currently quantitative RNA-Seq methods are pushed to work with increasingly small starting amounts of RNA that require PCR amplification to generate libraries. However, it is unclear how much noise or bias amplification introduces and how this effects precision and accuracy ...

Recent advances in RNA-seq methodologies from limiting amounts of mRNA have facilitated the characterization of rare cell-types in various biological systems. So far, however, technical variations in these methods have not been adequately characterized, vis-à-vis sensitivity, starting with reduced levels ...

Emerging methods for the accurate quantification of gene expression in individual cells hold promise for revealing the extent, function and origins of cell-to-cell variability. Different high-throughput methods for single-cell RNA-seq have been introduced that vary in coverage, sensitivity and multiplexing ...